Journal: ACS Infectious Diseases

Article Title: The SARS-CoV-2 Cytopathic Effect Is Blocked by Lysosome Alkalizing Small Molecules

doi: 10.1021/acsinfecdis.0c00349

Figure Lengend Snippet: CPE activity and toxicity for ROC-325, clomipramine, hycanthone, and verteporfin. (A) ROC-325, (B) clomipramine, (C) hycanthone, and (D) verteporfin CPE activity (blue curve, left graph) and toxicity (green curve, right graph) in 10 point, 1:2 dilution concentration–response curves starting at 30.0 μM down to 2.29 nM, along with their structures. ROC-325 started at 15 μM down to 1.14 nM. The red dashed line indicates EC 50 or CC 50 for CPE and toxicity assays, respectively. Duplicate values are shown for each concentration. Curves were generated using nonlinear regression.

Article Snippet: To demonstrate the day to day reproducibility of the CPE assay, compound concentration–response curves and EC 50 values from multiple experiments are shown for CQ, HCQ, and remdesivir ( Figure S2 ).

Techniques: Activity Assay, Concentration Assay, Generated

Journal: ACS Infectious Diseases

Article Title: The SARS-CoV-2 Cytopathic Effect Is Blocked by Lysosome Alkalizing Small Molecules

doi: 10.1021/acsinfecdis.0c00349

Figure Lengend Snippet: CPE activity and toxicity for CQ, HCQ, mefloquine, and remdesivir. (A) CQ, (B) HCQ, (C) mefloquine, and (D) remdesivir CPE activity (blue curve, left graph) and toxicity (green curve, right graph) in 10 point, 1:2 dilution concentration–response curves starting at 30.0 μM down to 2.29 nM, along with their structures. The dashed line indicates EC 50 or CC 50 for CPE and toxicity assays, respectively. Duplicate values are shown for each concentration. Curves were generated using nonlinear regression.

Article Snippet: To demonstrate the day to day reproducibility of the CPE assay, compound concentration–response curves and EC 50 values from multiple experiments are shown for CQ, HCQ, and remdesivir ( Figure S2 ).

Techniques: Activity Assay, Concentration Assay, Generated

Journal: ACS Infectious Diseases

Article Title: The SARS-CoV-2 Cytopathic Effect Is Blocked by Lysosome Alkalizing Small Molecules

doi: 10.1021/acsinfecdis.0c00349

Figure Lengend Snippet: CPE Assay in Vero E6 and Average LC3B-Based Autophagy Assay Parameters from Four Cell Lines a

Article Snippet: To demonstrate the day to day reproducibility of the CPE assay, compound concentration–response curves and EC 50 values from multiple experiments are shown for CQ, HCQ, and remdesivir ( Figure S2 ).

Techniques:

Journal: ACS Infectious Diseases

Article Title: The SARS-CoV-2 Cytopathic Effect Is Blocked by Lysosome Alkalizing Small Molecules

doi: 10.1021/acsinfecdis.0c00349

Figure Lengend Snippet: PP EC 50 and CC 50 Average Values with the SI (CC 50 /EC 50 Ratio) a

Article Snippet: To demonstrate the day to day reproducibility of the CPE assay, compound concentration–response curves and EC 50 values from multiple experiments are shown for CQ, HCQ, and remdesivir ( Figure S2 ).

Techniques: